FastQCFastQC Report
Wed 3 Dec 2025
Microbial-Standard-r1_S13_1000bp_run0_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMicrobial-Standard-r1_S13_1000bp_run0_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20275
Total Bases5.5 Mbp
Sequences flagged as poor quality0
Sequence length189-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2461.2133168927250308No Hit
GACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2261.1146732429099877No Hit
GACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2181.0752157829839704No Hit
GACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2141.055487053020962No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2121.0456226880394575No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2051.0110974106041923No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2041.0061652281134401No Hit
GACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1910.9420468557336621No Hit
GACTACCCGGGTGTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1860.9173859432799013No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1850.9124537607891493No Hit
GACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1850.9124537607891493No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1830.9025893958076449No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1800.8877928483353885No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1790.8828606658446363No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1780.8779284833538841No Hit
GACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1770.872996300863132No Hit
GACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1760.8680641183723797No Hit
GAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1740.8581997533908755No Hit
GACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1730.8532675709001233No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1720.8483353884093712No Hit
GACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1710.843403205918619No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1710.843403205918619No Hit
GACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1710.843403205918619No Hit
GACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1690.8335388409371147No Hit
GAATACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1680.8286066584463625No Hit
GACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1670.8236744759556103No Hit
GACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1670.8236744759556103No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1670.8236744759556103No Hit
GACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1660.8187422934648583No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1640.8088779284833539No Hit
GACTACCCGGGTGTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1620.7990135635018495No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1620.7990135635018495No Hit
GACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1610.7940813810110975No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1610.7940813810110975No Hit
GATTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1610.7940813810110975No Hit
GACTACAGGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1590.7842170160295932No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1560.7694204685573366No Hit
GACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1550.7644882860665845No Hit
GACTACAGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1540.7595561035758324No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1520.7496917385943279No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1500.7398273736128237No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1500.7398273736128237No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1480.7299630086313194No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1470.7250308261405671No Hit
GACTACCCGGGTGTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1440.7102342786683107No Hit
GACTACCGGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1420.7003699136868065No Hit
GACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1420.7003699136868065No Hit
GACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1390.6855733662145499No Hit
GACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1390.6855733662145499No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1380.6806411837237978No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1370.6757090012330457No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1370.6757090012330457No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1350.6658446362515413No Hit
GACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1350.6658446362515413No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1340.6609124537607891No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1340.6609124537607891No Hit
GACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1330.655980271270037No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1330.655980271270037No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1320.6510480887792849No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1310.6461159062885327No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1300.6411837237977805No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1290.6362515413070283No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1290.6362515413070283No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1280.6313193588162762No Hit
GACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1270.626387176325524No Hit
GAATACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1250.6165228113440198No Hit
GACTACCGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1250.6165228113440198No Hit
GACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1240.6115906288532676No Hit
GATTACCGGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1200.591861898890259No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1180.5819975339087546No Hit
GACTACAGGGGTCTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1180.5819975339087546No Hit
GACTACCCGGGTGTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1170.5770653514180024No Hit
GACTACCCGGGTGTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1160.5721331689272503No Hit
GACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1160.5721331689272503No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1160.5721331689272503No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1150.5672009864364982No Hit
GACTACCAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1150.5672009864364982No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1140.562268803945746No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1140.562268803945746No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1130.5573366214549939No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1120.5524044389642417No Hit
GAATACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1110.5474722564734895No Hit
GACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1110.5474722564734895No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1110.5474722564734895No Hit
GAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1100.5425400739827373No Hit
GACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1100.5425400739827373No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1090.5376078914919852No Hit
GATTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1080.5326757090012331No Hit
GACTACCAGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1080.5326757090012331No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1080.5326757090012331No Hit
GATTACCGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG1050.5178791615289766No Hit
GACTACCGGGGTCTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1040.5129469790382244No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1030.5080147965474723No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1020.5030826140567201No Hit
GACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1020.5030826140567201No Hit
GAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG990.48828606658446366No Hit
GACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG980.48335388409371144No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG970.47842170160295927No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG940.4636251541307028No Hit
GACTACCCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG930.45869297163995065No Hit
GACTACCCGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG920.45376078914919854No Hit
GACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG900.44389642416769426No Hit
GACTACCCGGGTGTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG900.44389642416769426No Hit
GATTACCGGGGTCTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG890.43896424167694204No Hit
GACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG880.43403205918618987No Hit
GACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG870.42909987669543775No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG870.42909987669543775No Hit
GACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG850.41923551171393336No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG820.40443896424167697No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG810.39950678175092474No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG790.38964241676942046No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG770.3797780517879162No Hit
GACTACCAGGGTCTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG760.37484586929716396No Hit
GACTACCCGGGTCTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG740.3649815043156597No Hit
GATTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG730.3600493218249075No Hit
GACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG730.3600493218249075No Hit
GACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG720.35511713933415534No Hit
GACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG670.33045622688039455No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG630.31072749691738594No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG630.31072749691738594No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG590.2909987669543773No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG570.281134401972873No Hit
GACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG560.27620221948212087No Hit
GACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG550.27127003699136865No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG520.2564734895191122No Hit
GACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG480.23674475955610358No Hit
GACTACCCGGGTGTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG470.2318125770653514No Hit
GACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG470.2318125770653514No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG460.22688039457459927No Hit
GACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG460.22688039457459927No Hit
GAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG460.22688039457459927No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG450.22194821208384713No Hit
GACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG450.22194821208384713No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG430.2120838471023428No Hit
GACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG430.2120838471023428No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG420.20715166461159062No Hit
GACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG390.19235511713933415No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG390.19235511713933415No Hit
GATTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG380.18742293464858198No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG330.16276202219482122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)