FastQCFastQC Report
Wed 3 Dec 2025
Microbial-Standard-r1_S13_1000bp_run0_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMicrobial-Standard-r1_S13_1000bp_run0_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20275
Total Bases6 Mbp
Sequences flagged as poor quality0
Sequence length249-301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC5692.806411837237978No Hit
CCTGCGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC5552.7373612823674476No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC4942.436498150431566No Hit
CCTATGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC4562.249075215782984No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4242.091245376078915No Hit
CCTACGGGAGACAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC4202.0715166461159065No Hit
CCTATGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3861.903822441430333No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3741.844636251541307No Hit
CCTGCGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3671.810110974106042No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3381.6670776818742294No Hit
CCTACGGGGTGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3321.6374845869297165No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3301.627620221948212No Hit
CCTACGGGAGACAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3151.5536374845869296No Hit
CCTACGGGAGCCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3021.4895191122071516No Hit
CCTATGGGGCGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2921.44019728729963No Hit
CCTACGGGGCGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2881.4204685573366214No Hit
TCTACGGAAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2721.3415536374845871No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2651.3070283600493218No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT2641.3020961775585698No Hit
CCTGCGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT2611.2872996300863133No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2581.2725030826140566No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2491.2281134401972873No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2391.1787916152897657No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT2381.1738594327990135No Hit
CCTATGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT2361.1639950678175093No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2361.1639950678175093No Hit
CCTACGGGGGCCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2361.1639950678175093No Hit
CCTGCGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2241.1048088779284835No Hit
TCTACGGTTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2221.094944512946979No Hit
CCTATGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2121.0456226880394575No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2101.0357583230579532No Hit
CCTACGGGAGACAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT2071.0209617755856966No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2071.0209617755856966No Hit
CCTACGGGAGCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2071.0209617755856966No Hit
CCTGCGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2061.0160295930949446No Hit
TCCACGGAGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2020.9963008631319359No Hit
CCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2000.9864364981504316No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1960.9667077681874229No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1930.9519112207151665No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1890.9321824907521579No Hit
TCTACGGAAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1890.9321824907521579No Hit
CCTACGGGAGACAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1760.8680641183723797No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1740.8581997533908755No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1640.8088779284833539No Hit
TCTACGGAAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1630.8039457459926017No Hit
CCTATGGGGCGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1630.8039457459926017No Hit
CCTACGGGGTGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1620.7990135635018495No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1620.7990135635018495No Hit
TCTACGGTTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1590.7842170160295932No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1580.7792848335388409No Hit
CCTATGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1560.7694204685573366No Hit
CCTACGGGAGCCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1550.7644882860665845No Hit
CCTACGGGGCGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1450.7151664611590629No Hit
CCTACGGGGTGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1430.7053020961775586No Hit
CCTACGGGGCGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1430.7053020961775586No Hit
CTTACGAGGCGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC1420.7003699136868065No Hit
TCCACGGAGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1410.6954377311960542No Hit
CCTACGGGGGCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1390.6855733662145499No Hit
CCTACGGGAGACAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1350.6658446362515413No Hit
CCTATGGGGCGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1290.6362515413070283No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1280.6313193588162762No Hit
CCTACGGGAGCCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1220.6017262638717632No Hit
CCTACGGGGGCCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1170.5770653514180024No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1150.5672009864364982No Hit
CCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1090.5376078914919852No Hit
CCTACGGGAGCCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1080.5326757090012331No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1060.5228113440197287No Hit
TCTACGGAAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1000.4932182490752158No Hit
TCTACGGTTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC980.48335388409371144No Hit
CCTACGGGGGCCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC970.47842170160295927No Hit
TCCACGGAGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC970.47842170160295927No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC960.47348951911220716No Hit
CCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC940.4636251541307028No Hit
TCTACGGTTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT920.45376078914919854No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT910.44882860665844637No Hit
TCCACGGAGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT890.43896424167694204No Hit
CTTACGAGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT890.43896424167694204No Hit
CCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC870.42909987669543775No Hit
CCTGCGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT870.42909987669543775No Hit
CTTACGAGGCGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC820.40443896424167697No Hit
CTTACGAGGCGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT800.39457459926017263No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC790.38964241676942046No Hit
TCTACGGAAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC760.37484586929716396No Hit
CCTACGGGGGCCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC750.36991368680641185No Hit
TCTACGGTTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC740.3649815043156597No Hit
CCTATGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT730.3600493218249075No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT640.3156596794081381No Hit
CCTACGGGAGACAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT630.31072749691738594No Hit
TCCACGGAGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC630.31072749691738594No Hit
CCTACGGGGCGCACCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC610.3008631319358816No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT600.2959309494451295No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT570.281134401972873No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT520.2564734895191122No Hit
CCTACGGGAGCCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT520.2564734895191122No Hit
CCTATGGGGCGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT500.2466091245376079No Hit
CTTACGAGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC470.2318125770653514No Hit
TCTACGGAAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT420.20715166461159062No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT400.19728729963008632No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTCCGCAATGGACGAAAGTCTGAC400.19728729963008632No Hit
CCTGCGGGAGGCAGCAGTAGGGAATATTCCGCAATGGACGAAAGTCTGAC380.18742293464858198No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT340.1676942046855734No Hit
CCTACGGGGGCCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT320.15782983970406905No Hit
CCTACGGGGCGCACCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT320.15782983970406905No Hit
TCCACGGAGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT310.1528976572133169No Hit
TCTACGGTTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT310.1528976572133169No Hit
CCTACGGGGCGCACCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT270.13316892725030827No Hit
CTTACGAGGCGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT240.11837237977805179No Hit
CCTACGGGGCGCACCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC220.10850801479654747No Hit
CCTATGGGAGGCAGCAGTAGGGAATATTCCGCAATGGACGAAAGTCTGAC220.10850801479654747No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTCCGCAATGGACGAAAGTCTGAC220.10850801479654747No Hit
CCTACGGGAGCCAGCAGTAGGGAATATTCCGCAATGGACGAAAGTCTGAC210.10357583230579531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)