Nextflow workflow report

[boring_poincare]

Workflow execution completed unsuccessfully!

The exit status of the task that caused the workflow execution to fail was: 1.

The full error message was:

Error executing process > 'NFCORE_MAG:MAG:BINNING_PREPARATION:SHORTREAD_BINNING_PREPARATION:BOWTIE2_ASSEMBLY_ALIGN (SPAdes-CR180008-CR180008)'

Caused by:
  Process `NFCORE_MAG:MAG:BINNING_PREPARATION:SHORTREAD_BINNING_PREPARATION:BOWTIE2_ASSEMBLY_ALIGN (SPAdes-CR180008-CR180008)` terminated with an error exit status (1)


Command executed:

  INDEX=`find -L ./ -name "*.rev.1.bt2l" -o -name "*.rev.1.bt2" | sed 's/.rev.1.bt2l//' | sed 's/.rev.1.bt2//'`
  bowtie2 \
      -p "2" \
      -x $INDEX \
       \
      -1 "CR180008_run0_phix_removed.unmapped_1.fastq.gz" -2 "CR180008_run0_phix_removed.unmapped_2.fastq.gz" \
      2> "SPAdes-CR180008-CR180008.bowtie2.log" |         samtools view -@ "2" -bS |         samtools sort -@ "2" -o "SPAdes-CR180008-CR180008.bam"
  samtools index "SPAdes-CR180008-CR180008.bam"
  
  if [ SPAdes-CR180008-CR180008 = "SPAdes-CR180008-CR180008" ] ; then
      mv "SPAdes-CR180008-CR180008.bowtie2.log" "SPAdes-CR180008.bowtie2.log"
  fi
  
  cat <<-END_VERSIONS > versions.yml
  "NFCORE_MAG:MAG:BINNING_PREPARATION:SHORTREAD_BINNING_PREPARATION:BOWTIE2_ASSEMBLY_ALIGN":
      bowtie2: $(echo $(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*$//')
      samtools: $(echo $(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*$//')
      pigz: $( pigz --version 2>&1 | sed 's/pigz //g' )
  END_VERSIONS

Command exit status:
  1

Command output:
  (empty)

Command error:
  view: invalid option -- '@'
  sort: invalid option -- '@'
  [main_samview] fail to open "2" for reading.
  open: No such file or directory
  [bam_sort_core] fail to open file 2

Work dir:
  /scratch1/liruiqi/meta_Osedax/mag_outputs/work/4e/b9ed6852bec91d92e8954832213e4b

Tip: when you have fixed the problem you can continue the execution adding the option `-resume` to the run command line
Run times
07-Feb-2026 17:41:33 - 07-Feb-2026 21:23:30 (duration: 3h 41m 57s)
  11 succeeded  
  0 cached  
  0 ignored  
  1 failed (0 retries)  
Nextflow command
nextflow run nf-core/mag -profile conda --input /scratch1/liruiqi/meta_Osedax/mag_outputs/samplesheet.csv --outdir /scratch1/liruiqi/meta_Osedax/mag_outputs --spades_fix_cpus 16 --host_fasta /scratch1/liruiqi/meta_Osedax/sylph_outputs/osedax_db/Osedax_frankpressi.fna --host_removal_verysensitive
CPU-Hours
53.3 (0% failed)
Launch directory
/scratch1/liruiqi/meta_Osedax/mag_outputs
Work directory
/scratch1/liruiqi/meta_Osedax/mag_outputs/work
Project directory
/home1/liruiqi/.nextflow/assets/nf-core/mag
Script name
main.nf
Script ID
1762f92e8d37b9d1e06d48c0f9275479
Workflow session
c2b0a69c-448c-4c05-90cf-c64349aebdc0
Workflow repository
https://github.com/nf-core/mag, revision main (commit hash b52fa53592ac9de93d3764ce69a05a711ae9f498)
Workflow profile
conda
Nextflow version
version 25.10.3, build 10983 (22-01-2026 15:34 UTC)

Resource Usage

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Job Duration

I/O

Tasks

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